Advanced Peptide Technology

The matrix is formed from synthetic peptides which are composed of three naturally occurring amino acids in a sequence which is repeated 4 times (Arginine - Alanine - Aspartic Acid - Alanine). The peptides self assemble to form a matrix upon a change in pH caused by exposure to an ions such as Na+ (sodium) or K+ (potassium). Each amino acid, when assembled into a peptide, will take part in creating a specific three dimensional shape. The amino acids are synthesized in a very specific order, and in doing so a very specific shape-forming capability will be programmed into the peptide.

The peptides in PuraStat are synthetized in a specific sequence which provide the molecule with shape memory to enable them to self assemble into a scaffold which mimics human extra cellular matrix (ECM). Based on results of scanning electron microscopy, the structure of PuraStat self-assembling peptide hydrogel is understood to be a network of fibres with a diameter of around 10–20 nm and pores with a diameter of about 50–200 nm that resembles the mesh structure of natural collagen.

1 Dhwani J., Rajesh V. A Review on Extracellular Matrix Mimicking Strategies for an Artificial Stem Cell Niche. Polymer Reviews. 2015, 55:4, 561-595, DOI:10.1080/15583724.2015.1040552